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XRN2 Recombinant antibody

XRN2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240065G4

Cat no : 83378-1-RR

Print datasheet

Synonyms

DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2

验证数据展示

  • WB analysis using 83378-1-RR

    WB analysis using 83378-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83378-1-RR (XRN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83378-1-RR

    FC experiment of HeLa using 83378-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug XRN2 Recombinant antibody (83378-1-RR, Clone:240065G4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83378-1-RR

    Affinity and Kinetic Characterization of 83378-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83378-1-RR against Human XRN2 were performed. The affinity constant is 1.49 nM.

经过测试的应用

Positive WB detected inMCF-7 cells, Jurkat cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy XRN2 Ready-To-Use IHC Kit. XRN2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83378-1-RR targets XRN2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen XRN2 fusion protein Ag30781 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5'-3' exoribonuclease 2
Synonyms DHM1-like protein, DHM1 like protein, 5'-3' exoribonuclease 2, 5' 3' exoribonuclease 2, 5 3 exoribonuclease 2
Calculated Molecular Weight 104 kDa
Observed Molecular Weight109 kDa
GenBank Accession NumberBC006417
Gene Symbol XRN2
Gene ID (NCBI) 22803
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H0D6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.

实验方案

Product Specific Protocols
WB protocol for XRN2 antibody 83378-1-RRDownload protocol
FC protocol for XRN2 antibody 83378-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols