验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, BxPC-3 cells |
| Positive IHC detected in | mouse kidney tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
55061-1-AP targets XYLT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | xylosyltransferase I |
| 别名 | Peptide O xylosyltransferase 1, XT I, XT1, Xylosyltransferase 1, xylosyltransferase I, XylT I, XYLT1 |
| 计算分子量 | 108 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | NM_022166 |
| 基因名称 | XYLT1 |
| Gene ID (NCBI) | 64131 |
| RRID | AB_10804758 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86Y38 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XYLT1, also named as XT1, Belongs to the glycosyltransferase 14 family and XylT subfamily. It catalyzes the first step in biosynthesis of glycosaminoglycan. XYLT1 transfers D-xylose from UDP-D-xylose to specific serine residues of the core protein. Initial enzyme in the biosynthesis of chondroitin sulfate and dermatan sulfate proteoglycans in fibroblasts and chondrocytes. The antibody is specific to XYLT1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XYLT1 antibody 55061-1-AP | Download protocol |
| IHC protocol for XYLT1 antibody 55061-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Phytomedicine Gua Lou Er Chen decoction attenuates atherosclerosis by reducing proteoglycans accumulation and inflammation | ||
J Biol Chem Chondroitin sulfate modification of CSPG4 regulates the maintenance and differentiation of glioma initiating cells via integrin-associated signaling |
