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YAF2 Polyclonal antibody

YAF2 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13980-1-AP

Print datasheet

Synonyms

YY1-associated factor 2, YY1 associated factor 2

验证数据展示

  • IP experiment of HeLa using 13980-1-AP

    IP experiment of HeLa using 13980-1-AP

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:600) with HeLa cells lysate 1760 ug.

  • IHC staining of mouse brain using 13980-1-AP

    IHC staining of mouse brain using 13980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13980-1-AP

    IHC staining of mouse brain using 13980-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 13980-1-AP

    IF Staining of U2OS using 13980-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YAF2 antibody (13980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13980-1-AP targets YAF2 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen YAF2 fusion protein Ag5023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YY1 associated factor 2
Synonyms YY1-associated factor 2, YY1 associated factor 2
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight20-30 kDa
GenBank Accession NumberBC037777
Gene Symbol YAF2
Gene ID (NCBI) 10138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

实验方案

Product Specific Protocols
IHC protocol for YAF2 antibody 13980-1-APDownload protocol
IF protocol for YAF2 antibody 13980-1-APDownload protocol
IP protocol for YAF2 antibody 13980-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols