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YAF2 Recombinant antibody

YAF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240834G11

Cat no : 83798-6-RR

Print datasheet

Synonyms

YY1-associated factor 2, YY1 associated factor 2, 240834G11

验证数据展示

  • WB analysis using 83798-6-RR

    WB analysis using 83798-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83798-6-RR (YAF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83798-6-RR

    FC experiment of HeLa using 83798-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug YAF2 Recombinant antibody (83798-6-RR, Clone:240834G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inHEK-293T cells, Raji cells, HeLa cells, SK-N-SH cells, SH-SY5Y cells, human heart tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83798-6-RR is YAF2 Fusion Protein expressed in E. coli.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen YAF2 fusion protein Ag5023 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name YY1 associated factor 2
Synonyms YY1-associated factor 2, YY1 associated factor 2, 240834G11
Calculated Molecular Weight 204 aa, 23 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC037777
Gene Symbol YAF2
Gene ID (NCBI) 10138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

实验方案

Product Specific Protocols
WB protocol for YAF2 antibody 83798-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols