YAP1 Polyclonal antibody
YAP1 Polyclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells |
Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30464-1-AP targets YAP1 in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, WB, ELISA |
Tested Reactivity | Human |
Immunogen | YAP1 fusion protein Ag33106 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | Yes-associated protein 1, 65kDa |
Synonyms | 65 kDa Yes associated protein, YAP, YAP1, YAP2, YAP65, YKI, Yorkie homolog |
Calculated Molecular Weight | 504 aa, 54 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC038235 |
Gene Symbol | YAP1 |
Gene ID (NCBI) | 10413 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P46937 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001).
实验方案
Product Specific Protocols | |
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WB protocol for YAP1 antibody 30464-1-AP | Download protocol |
IF protocol for YAP1 antibody 30464-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |