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YAP1 Recombinant antibody, PBS Only

YAP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E3

Cat no : 81090-1-PBS

Print datasheet

Synonyms

yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein

验证数据展示

  • WB analysis using 81090-1-RR (same clone as 81090-1-PBS)

    WB analysis using 81090-1-RR (same clone as 81090-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81090-1-RR (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 81090-1-RR (same clone as 81090-1-PBS)

    IHC staining of mouse skeletal muscle using 81090-1-RR (same clone as 81090-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YAP1 antibody included
  • IHC staining of mouse skeletal muscle using 81090-1-RR (same clone as 81090-1-PBS)

    IHC staining of mouse skeletal muscle using 81090-1-RR (same clone as 81090-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YAP1 antibody included
  • IHC staining of human colon cancer using 81090-1-RR (same clone as 81090-1-PBS)

    IHC staining of human colon cancer using 81090-1-RR (same clone as 81090-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YAP1 antibody included
  • IHC staining of human colon cancer using 81090-1-RR (same clone as 81090-1-PBS)

    IHC staining of human colon cancer using 81090-1-RR (same clone as 81090-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81090-1-RR (YAP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YAP1 antibody included
  • IF Staining of HepG2 using 81090-1-RR (same clone as 81090-1-PBS)

    IF Staining of HepG2 using 81090-1-RR (same clone as 81090-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YAP1 antibody (81090-1-RR, Clone: 5E3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81090-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81090-1-RR

    Affinity and Kinetic Characterization of 81090-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81090-1-RR against Human YAP1 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy YAP1 Ready-To-Use IHC Kit. YAP1 primary antibody included.

产品信息

81090-1-PBS targets YAP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen YAP1 fusion protein Ag4510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Yes-associated protein 1, 65kDa
Synonyms yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein
Calculated Molecular Weight 504 aa, 54 kDa
Observed Molecular Weight65-75 kDa
GenBank Accession NumberBC038235
Gene Symbol YAP1
Gene ID (NCBI) 10413
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP46937
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).