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YAP1 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IP, WB

Cat No : 61293,61294 61293

Synonyms

YAP1, Yes-associated protein 1, YAP, YKI, YAP2, YAP65, antibody, polyclonal, pAb, sample, western blotting, hippo, taz/wwtr1, taz, wwtr1, pax8, nkx2-1, ttft, smad, stem cell, stem, stem cells, immunoprecipitation, ip

验证数据展示

  • YAP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of YAP1 antibody was used to immunoprecipitate YAP1 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the YAP1 antibody at a dilution of 1:500.

    YAP1 antibody (pAb) tested by Immunoprecipitation. 10 ul of YAP1 antibody was used to immunoprecipitate YAP1 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the YAP1 antibody at a dilution of 1:500.

  • YAP1 antibody (pAb) tested by Western blot. YAP1 detection by Western blot. The analysis was performed using 40 ug HeLa whole-cell extract and YAP1 at a 1:500 dilution.

    YAP1 antibody (pAb) tested by Western blot. YAP1 detection by Western blot. The analysis was performed using 40 ug HeLa whole-cell extract and YAP1 at a 1:500 dilution.

产品信息

Tested Applications IP, WB

Applications Validated by Active Motif: IP: 10 ul per IP WB: 1:500 - 1:1,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This YAP1 antibody was raised against a peptide corresponding to the C-terminal region of human YAP1.
Full Name YAP1 antibody (pAb)
Synonyms YAP1, Yes-associated protein 1, YAP, YKI, YAP2, YAP65, antibody, polyclonal, pAb, sample, western blotting, hippo, taz/wwtr1, taz, wwtr1, pax8, nkx2-1, ttft, smad, stem cell, stem, stem cells, immunoprecipitation, ip
Molecular weight 75 kDa
GenBank accession numberNP_001123617
RRIDAB_2614990
Purification Method Affinity Purified
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

YAP1 (Yes-associated protein 1, YAP, YKI, YAP2, YAP65) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. Is a critical modulator of epidermal stem cell proliferation. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction.