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验证数据展示

  • WB analysis using 31228-1-AP

    WB analysis using 31228-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31228-1-AP (YARS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31228-1-AP

    IHC staining of human stomach cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 31228-1-AP

    IHC staining of human ovary cancer using 31228-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31228-1-AP (YARS antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

YARS Polyclonal antibody

YARS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31228-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, ELISA

别名

YARS1, YTS, YRS, TYRRS, CMTDIC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, U2OS cells, pig pancreas tissue, pig tonsil tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31228-1-AP targets YARS in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, pig
免疫原 YARS fusion protein Ag35148 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tyrosyl-tRNA synthetase
别名 YARS1, YTS, YRS, TYRRS, CMTDIC
计算分子量 59 kDa
观测分子量60 kDa
GenBank蛋白编号BC016689
基因名称 YARS
Gene ID (NCBI) 8565
RRIDAB_3669909
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP54577
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)

实验方案

Product Specific Protocols
WB protocol for YARS antibody 31228-1-APDownload protocol
IHC protocol for YARS antibody 31228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols