验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HepG2 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human testis tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17150-1-AP targets YARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | YARS2 fusion protein Ag9530 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tyrosyl-tRNA synthetase 2, mitochondrial |
别名 | Tyrosine--tRNA ligase, mitochondrial, Tyrosine tRNA ligase, mt TyrRS, EC:6.1.1.1, CGI-04 |
计算分子量 | 477 aa, 53 kDa |
观测分子量 | 45-50 kDa |
GenBank蛋白编号 | BC015625 |
基因名称 | YARS2 |
Gene ID (NCBI) | 51067 |
RRID | AB_2219263 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y2Z4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for YARS2 antibody 17150-1-AP | Download protocol |
IHC protocol for YARS2 antibody 17150-1-AP | Download protocol |
IF protocol for YARS2 antibody 17150-1-AP | Download protocol |
IP protocol for YARS2 antibody 17150-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |