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验证数据展示

  • IP experiment of K-562 using 17150-1-AP

    IP experiment of K-562 using 17150-1-AP

    IP result of anti-YARS2 (IP:17150-1-AP, 4ug; Detection:17150-1-AP 1:1000) with K-562 cells lysate 4400 ug.

  • WB analysis of K-562 using 17150-1-AP

    WB analysis of K-562 using 17150-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17150-1-AP (YARS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17150-1-AP

    IHC staining of human testis using 17150-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 17150-1-AP

    IHC staining of human testis using 17150-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17150-1-AP (YARS2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 17150-1-AP

    IF Staining of HepG2 using 17150-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YARS2 antibody (17150-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

YARS2 Polyclonal antibody

YARS2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 17150-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Tyrosine--tRNA ligase, mitochondrial, Tyrosine tRNA ligase, mt TyrRS, EC:6.1.1.1, CGI-04

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, HepG2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human placenta tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17150-1-AP targets YARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen YARS2 fusion protein Ag9530 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosyl-tRNA synthetase 2, mitochondrial
Synonyms Tyrosine--tRNA ligase, mitochondrial, Tyrosine tRNA ligase, mt TyrRS, EC:6.1.1.1, CGI-04
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC015625
Gene Symbol YARS2
Gene ID (NCBI) 51067
RRIDAB_2219263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Z4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for YARS2 antibody 17150-1-APDownload protocol
IHC protocol for YARS2 antibody 17150-1-APDownload protocol
IF protocol for YARS2 antibody 17150-1-APDownload protocol
IP protocol for YARS2 antibody 17150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols