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验证数据展示

  • WB analysis using 84993-4-RR (same clone as 84993-4-PBS)

    WB analysis using 84993-4-RR (same clone as 84993-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84993-4-RR (YBX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84993-4-RR (same clone as 84993-4-PBS)

    IF Staining of HeLa using 84993-4-RR (same clone as 84993-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YBX1 antibody (84993-4-RR, Clone: 242462F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84993-4-RR (same clone as 84993-4-PBS)

    IHC staining of human colon cancer using 84993-4-RR (same clone as 84993-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84993-4-RR (YBX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84993-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YBX1 antibody included

YBX1 Recombinant antibody, PBS Only

YBX1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84993-4-PBS

产品说明书

CloneNo. 242462F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

242462F12, CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy YBX1 Ready-To-Use IHC Kit. YBX1 primary antibody included.

产品信息

84993-4-PBS targets YBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 YBX1 fusion protein Ag14129 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Y box binding protein 1
别名 242462F12, CBF A, CBF-A, CCAAT-binding transcription factor I subunit A, DBPB
计算分子量 324 aa, 36 kDa
观测分子量 36-56 kDa
GenBank蛋白编号BC010430
基因名称 YBX1
Gene ID (NCBI) 4904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP67809
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Y box binding protein 1(YBX1) is one of cis-acting elements which has a role in regulation of the expression of HLA class II genes. YBX1 is great of importance in regulation of PTP1B expression. It can bind to splice sites mediate pre-mRNA alternative splicing regulation. Also, it involves the regulation of translation by modulation of the interaction between the mRNA and eukaryotic initiation factors. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation. The calculated molecular weight of YBX1 is 36 kDa, but modified YBX1 is about 36-56 kDa. (PMID: 27384875 )