验证数据展示
经过测试的应用
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13538-1-AP targets MSY2 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, chIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | MSY2 fusion protein Ag4465 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Y box binding protein 2 |
| 别名 | Germ cell-specific Y-box-binding protein, DNA-binding protein C, DNA binding protein C, Dbpc, CSDA3 |
| 计算分子量 | 364 aa, 39 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC033800 |
| 基因名称 | YBX2 |
| Gene ID (NCBI) | 51087 |
| RRID | AB_2241777 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2T7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MSY2, also named CSDA3 and YBX2, belongs to the Y box family of cold shock domain proteins. MSY2 is solely expressed in male and female germ cells and is necessary for fertility. MSY2 serves as a coactivator of transcription, binding to the DNA sequence CTGATTGGC/TC/TAA in the promoters of genes transcribed in germ cells, thereby linking transcription with mRNA storage/stabilization (PMID: 19597149). MSY2 has 1 isoform with a molecular mass of 38 kDa. MSY2 is essential for regulating mRNA stability in oocytes (PMID: 21613634).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MSY2 antibody 13538-1-AP | Download protocol |
| IHC protocol for MSY2 antibody 13538-1-AP | Download protocol |
| IP protocol for MSY2 antibody 13538-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Methylation in pericytes after acute injury promotes chronic kidney disease. | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis | ||
Development MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis. |
