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验证数据展示

  • IP experiment of A431 using 13977-1-AP

    IP experiment of A431 using 13977-1-AP

    IP result of anti-YES1 (IP:13977-1-AP, 4ug; Detection:13977-1-AP 1:300) with A431 cells lysate 2280 ug.

  • IHC staining of human breast cancer using 13977-1-AP

    IHC staining of human breast cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included
  • IHC staining of human breast cancer using 13977-1-AP

    IHC staining of human breast cancer using 13977-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13977-1-AP (YES1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with YES1 antibody included

YES1 Polyclonal antibody

YES1 Polyclonal Antibody for IP, IHC, ELISA
Cat No. 13977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IP, IHC, ELISA

别名

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IP detected inA431 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13977-1-AP targets YES1 in IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human
免疫原 YES1 fusion protein Ag5016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
别名 c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1
计算分子量 61 kDa
观测分子量 62 kDa
GenBank蛋白编号BC048960
基因名称 YES1
Gene ID (NCBI) 7525
RRIDAB_2877998
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP07947
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

实验方案

Product Specific Protocols
IHC protocol for YES1 antibody 13977-1-APDownload protocol
IP protocol for YES1 antibody 13977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols