• Featured Product
  • KD/KO Validated

YES1-Specific Polyclonal antibody

YES1-Specific Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20243-1-AP

Print datasheet

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1, YES1-Specific



经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, A549 cells, DU 145 cells, HEK-293 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 2 publications below
IHCSee 2 publications below

产品信息

20243-1-AP targets YES1-Specific in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IP, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Synonyms c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1, YES1-Specific
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberNM_005433
Gene Symbol YES1
Gene ID (NCBI) 7525
RRIDAB_10697656
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07947
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.

实验方案

Product Specific Protocols
WB protocol for YES1-Specific antibody 20243-1-APDownload protocol
IHC protocol for YES1-Specific antibody 20243-1-APDownload protocol
IP protocol for YES1-Specific antibody 20243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Respir Crit Care Med

YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib.

Authors - Irati Garmendia
human,mouse

Sci Adv

A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets.

Authors - Jackson A Roberts
humanIHC

J Immunother Cancer

SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.

Authors - Esther Redin
  • KO Validated
human

Cell Death Dis

Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p.

Authors - Yujing Sun
human

J Cell Biochem

miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.

Authors - Lan Li
  • KD Validated
human

iScience

DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer

Authors - Qingwen Huang