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YIPF5 Polyclonal antibody

YIPF5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12931-1-AP

Print datasheet

Synonyms

SB140, Protein YIPF5, PP12723, MEDS2, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5

验证数据展示

  • WB analysis using 12931-1-AP

    WB analysis using 12931-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12931-1-AP (YIPF5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 12931-1-AP

    IHC staining of human ovary cancer using 12931-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12931-1-AP (YIPF5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12931-1-AP targets YIPF5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen YIPF5 fusion protein Ag3802 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Yip1 domain family, member 5
Synonyms SB140, Protein YIPF5, PP12723, MEDS2, Five-pass transmembrane protein localizing in the Golgi apparatus and the endoplasmic reticulum 5
Calculated Molecular Weight 257 aa, 28 kDa
Observed Molecular Weight 28-33 kDa
GenBank Accession NumberBC024737
Gene Symbol YIPF5
Gene ID (NCBI) 81555
RRIDAB_2217209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969M3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for YIPF5 antibody 12931-1-APDownload protocol
IHC protocol for YIPF5 antibody 12931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols