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验证数据展示

  • WB analysis of HeLa using 66551-1-Ig

    WB analysis of HeLa using 66551-1-Ig

    WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells.

  • WB analysis using 66551-1-Ig

    WB analysis using 66551-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human kidney using 66551-1-Ig

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 66551-1-Ig

    IHC staining of human kidney using 66551-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

YME1L1 Monoclonal antibody

YME1L1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66551-1-Ig

产品说明书

CloneNo. 2B7E4

宿主/亚型

Mouse / IgG1

种属反应性

Human, Rat, mouse

应用

WB, IHC, ELISA

别名

FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells, THP-1 cells, HSC-T6 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66551-1-Ig targets YME1L1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Rat, mouse
免疫原 YME1L1 fusion protein Ag2069 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 YME1-like 1 (S. cerevisiae)
别名 FTSH, FTSH1, Meg 4, MEG4, PAMP, YME1 like 1 (S. cerevisiae), YME1 like protein 1, YME1L, YME1L1
计算分子量 716 aa, 80 kDa
观测分子量 63 kDa
GenBank蛋白编号BC023507
基因名称 YME1L1
Gene ID (NCBI) 10730
RRIDAB_2881913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96TA2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

实验方案

Product Specific Protocols
WB protocol for YME1L1 antibody 66551-1-IgDownload protocol
IHC protocol for YME1L1 antibody 66551-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols