验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25370-1-AP targets YOD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, pig, monkey |
| 免疫原 | YOD1 fusion protein Ag21881 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) |
| 别名 | DUBA 8, DUBA8, HIN 7, HIN7, HIV 1 induced protease 7, HsHIN7, OTUD2, PRO0907, Ubiquitin thioesterase OTU1, YOD1 |
| 计算分子量 | 348 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC137167 |
| 基因名称 | YOD1 |
| Gene ID (NCBI) | 55432 |
| RRID | AB_2880048 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5VVQ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YOD1 antibody 25370-1-AP | Download protocol |
| IHC protocol for YOD1 antibody 25370-1-AP | Download protocol |
| IP protocol for YOD1 antibody 25370-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Comput Struct Biotechnol J Identification of potential diagnostic and prognostic biomarkers for sepsis based on machine learning | ||
Mol Cell Proteomics The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators | ||
Cell Signal Hypoxic tumor-derived exosomal miR-21 induces cancer-associated fibroblast activation to promote head and neck squamous cell carcinoma metastasis | ||
Biomed Res Int Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-β3 Signaling Pathway.
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