验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84933-5-RR targets YOD1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | YOD1 fusion protein Ag21844 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae) |
| 别名 | 242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12 |
| 计算分子量 | 348 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC137167 |
| 基因名称 | YOD1 |
| Gene ID (NCBI) | 55432 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q5VVQ6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YOD1 antibody 84933-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |