验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, MDA-MB-231 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
29441-1-AP targets YTHDC1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, IP, RIP |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | YTHDC1 fusion protein Ag31459 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | YTH domain containing 1 |
| Synonyms | KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1 |
| Calculated Molecular Weight | 85 kDa |
| Observed Molecular Weight | ~100 kDa |
| GenBank Accession Number | BC053863 |
| Gene Symbol | YTHDC1 |
| Gene ID (NCBI) | 91746 |
| RRID | AB_2918307 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MU7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDC1 antibody 29441-1-AP | Download protocol |
| IHC protocol for YTHDC1 antibody 29441-1-AP | Download protocol |
| IP protocol for YTHDC1 antibody 29441-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Int YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.
| ||
J Clin Lab Anal Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling. | ||
Nanoscale Res Lett RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation. | ||
Discov Oncol Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma | ||
Cell Stem Cell Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance | ||
Exp Mol Med The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression
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