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YTHDC1 Polyclonal antibody

YTHDC1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 29441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat and More (1)

应用

WB, IP, IHC, ELISA and More (2)

KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, MDA-MB-231 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy YTHDC1 Ready-To-Use IHC Kit. YTHDC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29441-1-AP targets YTHDC1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP, RIP
经测试反应性 Human, mouse, rat
文献引用反应性human, mouse
免疫原 YTHDC1 fusion protein Ag31459 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YTH domain containing 1
别名 KIAA1966, Putative splicing factor YT521, YT521, YT521 B, YTH domain containing 1, YTHDC1
计算分子量 85 kDa
观测分子量~100 kDa
GenBank蛋白编号BC053863
基因名称 YTHDC1
Gene ID (NCBI) 91746
RRIDAB_2918307
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MU7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

实验方案

Product Specific Protocols
WB protocol for YTHDC1 antibody 29441-1-APDownload protocol
IHC protocol for YTHDC1 antibody 29441-1-APDownload protocol
IP protocol for YTHDC1 antibody 29441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

YTHDC1-mediated VPS25 regulates cell cycle by targeting JAK-STAT signaling in human glioma cells.

Authors - Xiaolong Zhu
  • KD Validated
mouseWB

J Clin Lab Anal

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Authors - Zhenwei Li
humanWB

Nanoscale Res Lett

RNA m6A Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation.

Authors - Jinbin Pan
humanWB

Discov Oncol

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma

Authors - Fanhua Kong
humanRIP

Cell Stem Cell

Decoding m6A RNA methylome identifies PRMT6-regulated lipid transport promoting AML stem cell maintenance

Authors - Ying Cheng
humanWB,IHC,IP

Exp Mol Med

The YTHDC1/GLUT3/RNF183 axis forms a positive feedback loop that modulates glucose metabolism and bladder cancer progression

Authors - Bin Yan
  • KD Validated
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