验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IP detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26787-1-AP targets YTHDF1 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | YTHDF1 fusion protein Ag25347 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | YTH domain family, member 1 |
| 别名 | C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1 |
| 计算分子量 | 559 aa, 61 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC050284 |
| 基因名称 | YTHDF1 |
| Gene ID (NCBI) | 54915 |
| RRID | AB_2880635 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BYJ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF1 antibody 26787-1-AP | Download protocol |
| IP protocol for YTHDF1 antibody 26787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis | ||
Cell Mol Neurobiol Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord | ||
Cancer Med BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer | ||
Cancer Cell Int DUSP5 regulated by YTHDF1-mediated m6A modification promotes epithelial-mesenchymal transition and EGFR-TKI resistance via the TGF-β/Smad signaling pathway in lung adenocarcinoma
| ||
BMC Med YTHDF1 in periaqueductal gray inhibitory neurons contributes to morphine withdrawal responses in mice |