YTHDF1 Polyclonal antibody

YTHDF1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26787-1-AP

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Synonyms

C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1



经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26787-1-AP targets YTHDF1 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, RIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen YTHDF1 fusion protein Ag25347 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name YTH domain family, member 1
Synonyms C20orf21, DACA 1, YTH domain family protein 1, YTH domain family, member 1, YTHDF1
Calculated Molecular Weight 559 aa, 61 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC050284
Gene Symbol YTHDF1
Gene ID (NCBI) 54915
RRIDAB_2880635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF1, also named YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at the internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing, and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoforms and the calculated molecular weight of isoforms are 61 kDa and 21 kDa.

实验方案

Product Specific Protocols
WB protocol for YTHDF1 antibody 26787-1-APDownload protocol
IP protocol for YTHDF1 antibody 26787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,RIP,IHC

Cancer Res

RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis

Authors - Danyu Chen
mouseWB,IHC

Cell Mol Neurobiol

Wnt3a/YTHDF1 Regulated Oxaliplatin-Induced Neuropathic Pain Via TNF-α/IL-18 Expression in the Spinal Cord

Authors - Xiaohui Bai
humanWB

Cancer Med

BIRC5 facilitates cisplatin-chemoresistance in a m6 A-dependent manner in ovarian cancer

Authors - Yadan Fan
humanWB

Cancer Cell Int

DUSP5 regulated by YTHDF1-mediated m6A modification promotes epithelial-mesenchymal transition and EGFR-TKI resistance via the TGF-β/Smad signaling pathway in lung adenocarcinoma

Authors - Weina Fan
  • KD Validated
mouseWB,IHC

BMC Med

YTHDF1 in periaqueductal gray inhibitory neurons contributes to morphine withdrawal responses in mice

Authors - Chaopeng Ou