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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81340-1-RR (same clone as 81340-1-PBS)

    WB analysis using 81340-1-RR (same clone as 81340-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81340-1-RR (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81340-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 81340-1-RR (same clone as 81340-1-PBS)

    WB analysis of Jurkat using 81340-1-RR (same clone as 81340-1-PBS)

    WB result of YTHDF2 antibody (81340-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF2 transfected Jurkat cells. This data was developed using the same antibody clone with 81340-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 81340-1-RR (same clone as 81340-1-PBS)

    IP experiment of HeLa using 81340-1-RR (same clone as 81340-1-PBS)

    IP result of anti-YTHDF2 (IP:81340-1-RR, 4ug; Detection:81340-1-RR 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 81340-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 81340-1-RR (same clone as 81340-1-PBS)

    IF Staining of HeLa using 81340-1-RR (same clone as 81340-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (81340-1-RR, Clone: 3L6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81340-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81340-1-RR

    Affinity and Kinetic Characterization of 81340-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81340-1-RR against Human YTHDF2 were performed. The affinity constant is 41.6 pM.

  • Featured Product
  • KD/KO验证

YTHDF2 Recombinant antibody, PBS Only

YTHDF2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 81340-1-PBS

产品说明书

CloneNo. 3L6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14, CLL associated antigen KW 14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.

产品信息

81340-1-PBS targets YTHDF2 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YTHDF2 fusion protein Ag5922 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain family, member 2
别名 NY REN 2, HGRG8, DF2, CLL-associated antigen KW-14, CLL associated antigen KW 14
计算分子量 62 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC002559
基因名称 YTHDF2
Gene ID (NCBI) 51441
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5A9
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.