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验证数据展示

  • WB analysis using 84709-3-RR

    WB analysis using 84709-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84709-3-RR

    Affinity and Kinetic Characterization of 84709-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

YTHDF3 Recombinant antibody

YTHDF3 Recombinant Antibody for WB, ELISA
Cat No. 84709-3-RR

产品说明书

CloneNo. 242149F3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

YTH domain-containing family protein 3, DF3, 242149F3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YTHDF3 fusion protein Ag22407 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YTH domain family, member 3
别名 YTH domain-containing family protein 3, DF3, 242149F3
计算分子量 585 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC052970
基因名称 YTHDF3
Gene ID (NCBI) 253943
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z739
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

实验方案

Product Specific Protocols
WB protocol for YTHDF3 antibody 84709-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols