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YTHDF3 Recombinant antibody

YTHDF3 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242149F3

Cat no : 84709-3-RR

Print datasheet

Synonyms

YTH domain-containing family protein 3, DF3, 242149F3

验证数据展示

  • WB analysis using 84709-3-RR

    WB analysis using 84709-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84709-3-RR

    Affinity and Kinetic Characterization of 84709-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

经过测试的应用

Positive WB detected inHCT 116 cells, PC-12 cells, C6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF3 Ready-To-Use IHC Kit. YTHDF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84709-3-RR targets YTHDF3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen YTHDF3 fusion protein Ag22407 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name YTH domain family, member 3
Synonyms YTH domain-containing family protein 3, DF3, 242149F3
Calculated Molecular Weight 585 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z739
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.

实验方案

Product Specific Protocols
WB protocol for YTHDF3 antibody 84709-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols