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  • Featured Product
  • KD/KO Validated

YWHAH Polyclonal antibody

YWHAH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15222-1-AP

Print datasheet

Synonyms

14-3-3 eta, 14-3-3 protein eta, 14-3-3, 14 3 3 protein eta

验证数据展示

  • WB analysis of human brain using 15222-1-AP

    WB analysis of human brain using 15222-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15222-1-AP (YWHAH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15222-1-AP

    IF Staining of HeLa using 15222-1-AP

    Immunofluorescent analysis of Hela cells, using YWHAH antibody 15222-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of NIH/3T3 using 15222-1-AP

    IP experiment of NIH/3T3 using 15222-1-AP

    IP result of anti-YWHAH (IP:15222-1-AP, 4ug; Detection:15222-1-AP 1:1000) with NIH/3T3 cells lysate 1600ug.

  • IHC staining of human skin cancer using 15222-1-AP

    IHC staining of human skin cancer using 15222-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15222-1-AP (YWHAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 15222-1-AP

    IHC staining of human stomach using 15222-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15222-1-AP (YWHAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman brain tissue, A431 cells, mouse brain tissue, NIH/3T3 cells, Raji cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15222-1-AP targets YWHAH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen YWHAH fusion protein Ag7435 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
Synonyms 14-3-3 eta, 14-3-3 protein eta, 14-3-3, 14 3 3 protein eta
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC003047
Gene Symbol YWHAH
Gene ID (NCBI) 7533
RRIDAB_10863119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04917
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.

实验方案

Product Specific Protocols
WB protocol for YWHAH antibody 15222-1-APDownload protocol
IHC protocol for YWHAH antibody 15222-1-APDownload protocol
IF protocol for YWHAH antibody 15222-1-APDownload protocol
IP protocol for YWHAH antibody 15222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Circulating Exosomal MiR-107 Restrains Tumorigenesis in Diffuse Large B-Cell Lymphoma by Targeting 14-3-3η.

Authors - Jiarui Liu
View Article
humanWB,IF

Cell Biol Int

14-3-3-η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage

Authors - Hui Zhou
View Article
ratWB

Eur J Pharmacol

Capsaicin prevents mitochondrial damage, protects cardiomyocytes subjected to anoxia/reoxygenation injury mediated by 14-3-3η/Bcl-2.

Authors - Jiyi Huang
View Article
human,mouseWB,IF,IP

J Exp Clin Cancer Res

14-3-3ζ inhibits heme oxygenase-1 (HO-1) degradation and promotes hepatocellular carcinoma proliferation: involvement of STAT3 signaling.

Authors - Jia Song
  • KD Validated
View Article
ratWB

Cells

Identification of YWHAH as a Novel Brain-Derived Extracellular Vesicle Marker Post Long-Term Midazolam Exposure during Early Development

Authors - Nghi M Nguyen
View Article
humanIF

J Hazard Mater

YTHDC2 mediated RNA m6A modification contributes to PM2.5-induced hepatic steatosis

Authors - Zhipeng Yan
View Article