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YWHAZ Monoclonal antibody, PBS Only

YWHAZ Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat no : 67698-1-PBS

Print datasheet

Synonyms

14 3 3 protein zeta/delta, KCIP 1, YWHAZ

验证数据展示

  • WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

    WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67698-1-Ig (YWHAZ antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAZ antibody included
  • IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

    IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using YWHAZ antibody (67698-1-Ig, Clone: 3G12B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy YWHAZ Ready-To-Use IHC Kit. YWHAZ primary antibody included.

产品信息

67698-1-PBS targets YWHAZ in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen YWHAZ fusion protein Ag6667 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Synonyms 14 3 3 protein zeta/delta, KCIP 1, YWHAZ
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC003623
Gene Symbol YWHAZ
Gene ID (NCBI) 7534
RRIDAB_2882890
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP63104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.