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YWHAZ Monoclonal antibody, PBS Only

YWHAZ Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3G12B10

Cat No : 67698-1-PBS

Print datasheet

Synonyms

14 3 3 protein zeta/delta, KCIP 1, YWHAZ

验证数据展示

  • WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

    WB analysis using 67698-1-Ig (same clone as 67698-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67698-1-Ig (YWHAZ antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    IHC staining of human lung cancer using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67698-1-Ig (YWHAZ antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAZ antibody included
  • IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

    IF Staining of MCF-7 using 67698-1-Ig (same clone as 67698-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using YWHAZ antibody (67698-1-Ig, Clone: 3G12B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67698-1-PBS in a different storage buffer formulation.

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy YWHAZ Ready-To-Use IHC Kit. YWHAZ primary antibody included.

产品信息

67698-1-PBS targets YWHAZ in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen YWHAZ fusion protein Ag6667 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
Synonyms 14 3 3 protein zeta/delta, KCIP 1, YWHAZ
Calculated Molecular Weight 28 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC003623
Gene Symbol YWHAZ
Gene ID (NCBI) 7534
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP63104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.