- Featured Product
- KD/KO Validated
ZAK Polyclonal antibody
ZAK Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, human heart tissue, HepG2 cells, human skeletal muscle tissue |
| Positive IHC detected in | human liver cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
产品信息
14945-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ZAK fusion protein Ag6747 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sterile alpha motif and leucine zipper containing kinase AZK |
| Synonyms | AZK, HCCS 4, MAP3K20, Mitogen-activated protein kinase kinase kinase 20, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK |
| Calculated Molecular Weight | 91 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC001401 |
| Gene Symbol | ZAK |
| Gene ID (NCBI) | 51776 |
| RRID | AB_10642698 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYL2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZAK antibody 14945-1-AP | Download protocol |
| IHC protocol for ZAK antibody 14945-1-AP | Download protocol |
| IF protocol for ZAK antibody 14945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.
| ||
Sci Signal Poly-GR repeats associated with ALS/FTD gene C9ORF72 impair translation elongation and induce a ribotoxic stress response in neurons | ||
bioRxiv Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L | ||
Nat Commun Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation | ||
Cell Metab Ribosome stalling is a signal for metabolic regulation by the ribotoxic stress response | ||
Cell Death Dis Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanisms |
