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验证数据展示

  • WB analysis using 28761-1-AP

    WB analysis using 28761-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28761-1-AP (ZAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28761-1-AP

    WB analysis of HeLa using 28761-1-AP

    WB result of ZAK antibody (28761-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZAK transfected HeLa cells.

  • IHC staining of human colon cancer using 28761-1-AP

    IHC staining of human colon cancer using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 28761-1-AP

    IHC staining of human colon cancer using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse tongue using 28761-1-AP

    IHC staining of mouse tongue using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat tongue using 28761-1-AP

    IHC staining of rat tongue using 28761-1-AP

    Immunohistochemical analysis of paraffin-embedded rat tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 28761-1-AP

    IF Staining of HepG2 using 28761-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (28761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ZAK Polyclonal antibody

ZAK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28761-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

MAP3K20, Leucine zipper- and sterile alpha motif-containing kinase, HCCS-4, HCCS 4, EC:2.7.11.25

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, MDA-MB-231 cells
Positive IHC detected inhuman colon cancer tissue, rat tongue tissue, mouse tongue tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28761-1-AP targets ZAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ZAK fusion protein Ag30599 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sterile alpha motif and leucine zipper containing kinase AZK
别名 MAP3K20, Leucine zipper- and sterile alpha motif-containing kinase, HCCS-4, HCCS 4, EC:2.7.11.25
计算分子量 91 kDa
观测分子量91kDa, 51 kDa
GenBank蛋白编号BC001401
基因名称 ZAK
Gene ID (NCBI) 51776
RRIDAB_2918199
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYL2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

实验方案

Product Specific Protocols
WB protocol for ZAK antibody 28761-1-APDownload protocol
IHC protocol for ZAK antibody 28761-1-APDownload protocol
IF protocol for ZAK antibody 28761-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genome Biol

The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model

Authors - Yunfei Li
  • KD Validated
View Article
humanWB

J Cell Mol Med

Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals

Authors - Weiming Ma
View Article