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验证数据展示

  • WB analysis of Jurkat using 22207-1-AP

    WB analysis of Jurkat using 22207-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22207-1-AP (ZAP70 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 22207-1-AP

    IF Staining of RAW 264.7 using 22207-1-AP

    Immunofluorescent analysis of RAW 264.7 cells using 22207-1-AP (ZAP70 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of Jurkat using 22207-1-AP

    IP experiment of Jurkat using 22207-1-AP

    IP result of anti-ZAP70 (IP:22207-1-AP, 4ug; Detection:22207-1-AP 1:8000) with Jurkat cells lysate 1320 ug.

ZAP70 Polyclonal antibody

ZAP70 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 22207-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, RAW 264.7 cells, RAW264.7
Positive IP detected inJurkat cells
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22207-1-AP targets ZAP70 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 ZAP70 fusion protein Ag17527 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zeta-chain (TCR) associated protein kinase 70kDa
别名 Syk-related tyrosine kinase, STD, SRK, EC:2.7.10.2, 70 kDa zeta-chain associated protein
计算分子量 619 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC039039
基因名称 ZAP70
Gene ID (NCBI) 7535
ENSEMBL Gene IDENSG00000115085
RRIDAB_2879029
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP43403
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZAP70 antibody 22207-1-APDownload protocol
IF protocol for ZAP70 antibody 22207-1-APDownload protocol
IP protocol for ZAP70 antibody 22207-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols