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ZBP1 Polyclonal antibody
ZBP1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13285-1-AP targets ZBP1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | ZBP1 fusion protein Ag4119 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Z-DNA binding protein 1 |
| Synonyms | C20orf183, DAI, DLM 1, DLM1, Z DNA binding protein 1, ZBP1 |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 42-68 kDa |
| GenBank Accession Number | BC028218 |
| Gene Symbol | ZBP1 |
| Gene ID (NCBI) | 81030 |
| RRID | AB_2122651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H171 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZBP1 antibody 13285-1-AP | Download protocol |
| IHC protocol for ZBP1 antibody 13285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Mitochondrial DNA damage and subsequent activation of Z-DNA binding protein 1 links oxidative stress to inflammation in epithelial cells.
| ||
Physiol Genomics PANoptosis in vascular smooth muscle cells regulated by TNF-α/IL-1β can be a new target for alleviating the progression of abdominal aortic aneurysm | ||
J Biol Chem Oxidative stress induces Z-DNA-binding protein 1-dependent activation of microglia via mtDNA released from retinal pigment epithelial cells. | ||
Toxicol Appl Pharmacol The anti-cancer drug candidate CBL0137 induced necroptosis via forming left-handed Z-DNA and its binding protein ZBP1 in liver cells | ||
mBio Sperm-Associated Antigen 9 Promotes Influenza A Virus-Induced Cell Death via the c-Jun N-Terminal Kinase Signaling Pathway. | ||
Heliyon Analysis of the role of PANoptosis in seizures via integrated bioinformatics analysis and experimental validation |