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验证数据展示

  • IHC staining of human colon cancer using 84396-2-RR

    IHC staining of human colon cancer using 84396-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84396-2-RR (ZBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84396-2-RR

    IF Staining of HeLa using 84396-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84396-2-RR

    Affinity and Kinetic Characterization of 84396-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

ZBP1 Recombinant antibody

ZBP1 Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84396-2-RR

产品说明书

CloneNo. 241582E2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84396-2-RR targets ZBP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 ZBP1 fusion protein Ag4119 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Z-DNA binding protein 1
别名 Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183
计算分子量 46 kDa
GenBank蛋白编号BC028218
基因名称 ZBP1
Gene ID (NCBI) 81030
RRIDAB_3671927
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H171
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

实验方案

Product Specific Protocols
IHC protocol for ZBP1 antibody 84396-2-RRDownload protocol
IF protocol for ZBP1 antibody 84396-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols