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验证数据展示

  • IHC staining of human colon cancer using 84396-2-RR

    IHC staining of human colon cancer using 84396-2-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84396-2-RR (ZBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 84396-2-RR

    IF Staining of HeLa using 84396-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84396-2-RR

    Affinity and Kinetic Characterization of 84396-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

ZBP1 Recombinant antibody

ZBP1 Recombinant Antibody for IHC, IF/ICC, ELISA
Cat No. 84396-2-RR

Print datasheet

CloneNo. 241582E2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84396-2-RR targets ZBP1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ZBP1 fusion protein Ag4119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Z-DNA binding protein 1
Synonyms Tumor stroma and activated macrophage protein DLM-1, DLM1, DLM 1, DAI, C20orf183
Calculated Molecular Weight 46 kDa
GenBank Accession NumberBC028218
Gene Symbol ZBP1
Gene ID (NCBI) 81030
RRIDAB_3671927
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9H171
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

实验方案

Product Specific Protocols
IHC protocol for ZBP1 antibody 84396-2-RRDownload protocol
IF protocol for ZBP1 antibody 84396-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols