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ZBTB10 Polyclonal antibody

ZBTB10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22944-1-AP

Print datasheet

Synonyms

RINZF, RINZFC, ZBTB10, Zinc finger protein RIN ZF

验证数据展示

  • IP experiment of mouse liver using 22944-1-AP

    IP experiment of mouse liver using 22944-1-AP

    IP result of anti-ZBTB10 (IP:22944-1-AP, 3ug; Detection:22944-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

  • WB analysis of A375 using 22944-1-AP

    WB analysis of A375 using 22944-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 22944-1-AP (ZBTB10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA375 cells
Positive IP detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22944-1-AP is ZBTB10 Fusion Protein expressed in E. coli.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ZBTB10 fusion protein Ag19094 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 10
Synonyms RINZF, RINZFC, ZBTB10, Zinc finger protein RIN ZF
Calculated Molecular Weight 871 aa, 95 kDa
Observed Molecular Weight 65 kDa, 130 kDa
GenBank Accession NumberBC127098
Gene Symbol ZBTB10
Gene ID (NCBI) 65986
RRIDAB_2879188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96DT7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc finger and BTB domain-containing protein 10 (ZBTB10), also known as RINZF, RINZFC, May be involved in transcriptional regulation. Multiple isoforms of ZBTB10 exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for ZBTB10 antibody 22944-1-APDownload protocol
IP protocol for ZBTB10 antibody 22944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols