• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ZBTB20 Polyclonal antibody

ZBTB20 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23987-1-AP

Print datasheet

Synonyms

DPZF, HOF, ODA 8S, ZBTB20, Zinc finger protein 288, ZNF288

验证数据展示

  • WB analysis using 23987-1-AP

    WB analysis using 23987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 23987-1-AP

    WB analysis of L02 using 23987-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23987-1-AP (ZBTB20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 23987-1-AP

    IHC staining of human kidney using 23987-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23987-1-AP (ZBTB20 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ZBTB20 antibody included
  • IF Staining of mouse brain using 23987-1-AP

    IF Staining of mouse brain using 23987-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ZBTB20 antibody (23987-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HEK-293 cells, Jurkat cells, L02 cells, mouse brain tissue, mouse liver tissue, RAW 264.7 cells, rat liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy ZBTB20 Ready-To-Use IHC Kit. ZBTB20 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23987-1-AP targets ZBTB20 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ZBTB20 fusion protein Ag21140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 20
Synonyms DPZF, HOF, ODA 8S, ZBTB20, Zinc finger protein 288, ZNF288
Calculated Molecular Weight 668 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC029041
Gene Symbol ZBTB20
Gene ID (NCBI) 26137
RRIDAB_2879389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9HC78
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZBTB20 (zinc finger and BTB domain containing 20), also known as HOF, DPZF, ODA-8S or ZNF288, is a 741 amino acid protein that localizes to the nucleus and contains one BTB (POZ) domain and five C2H2-type zinc fingers. Expressed in thymus, spleen, lymph node and fetal liver, ZBTB20 exists as either a monomer or a homodimer that is thought to function as a transcription factor, playing a role in hematopoiesis, oncogenesis and immune responses. Multiple isoforms of ZBTB20 exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for ZBTB20 antibody 23987-1-APDownload protocol
IHC protocol for ZBTB20 antibody 23987-1-APDownload protocol
IF protocol for ZBTB20 antibody 23987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Selective modulation of the human glucocorticoid receptor compromises GR chromatin occupancy and recruitment of p300/CBP and the Mediator complex

Authors - Laura Van Moortel
  • KD Validated
View Article
IF

Cell Prolif

In vivo development and single-cell transcriptome profiling of human brain organoids.

Authors - Shichao Huang
View Article
mouseWB,IHC

J Inflamm Res

Aberrant Fluid Shear Stress Contributes to Articular Cartilage Pathogenesis via Epigenetic Regulation of ZBTB20 by H3K4me3.

Authors - Yu Jin
View Article
mouseWB

Wellcome Open Res

The integration site of the APP transgene in the J20 mouse model of Alzheimer's disease.

Authors - Justin L Tosh
View Article
ratWB

Cell Rep

Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.

Authors - Eva Riechert
View Article
mouseWB

Transpl Immunol

ZBTB20 regulates cardiac allograft rejection through NFкB-mediated inflammation in mouse heart transplantation.

Authors - Liuyi Lu
View Article