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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 12516-1-AP (same clone as 12516-1-PBS)

    WB analysis using 12516-1-AP (same clone as 12516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12516-1-AP (ZBTB32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12516-1-AP (same clone as 12516-1-PBS)

    IF Staining of MCF-7 using 12516-1-AP (same clone as 12516-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 21738-1-AP 00069062 (red). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

ZBTB32 Polyclonal antibody, PBS Only

ZBTB32 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 12516-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

12516-1-PBS targets ZBTB32 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ZBTB32 fusion protein Ag3199 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 32
别名
计算分子量 487 aa, 53 kDa
观测分子量53 kDa
GenBank蛋白编号BC017700
基因名称 ZBTB32
Gene ID (NCBI) 27033
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2Y4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The zinc finger and BTB domain containing 32 (ZBTB32) protein, also known as testis zinc finger protein (TZFP) or repressor of GATA (ROG), is a transcription factor belonging to the BTB/POZ-ZF protein family. It is encoded by the Zbtb32 gene, located on proximal chromosome 7 in the mouse genome. ZBTB32 was shown to ensure proper regulation of meiosis during spermatogenesis by acting as a repressor of the androgen receptor (PMID: 22851713, 34337361).