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ZBTB32 Polyclonal antibody, PBS Only

ZBTB32 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12516-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 12516-1-AP (same clone as 12516-1-PBS)

    WB analysis using 12516-1-AP (same clone as 12516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12516-1-AP (ZBTB32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    IHC staining of mouse testis using 12516-1-AP (same clone as 12516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12516-1-AP (ZBTB32 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 12516-1-AP (same clone as 12516-1-PBS)

    IF Staining of MCF-7 using 12516-1-AP (same clone as 12516-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB32 antibody (12516-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 21738-1-AP 00069062 (red). This data was developed using the same antibody clone with 12516-1-PBS in a different storage buffer formulation.

产品信息

12516-1-PBS targets ZBTB32 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ZBTB32 fusion protein Ag3199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 32
Synonyms
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC017700
Gene Symbol ZBTB32
Gene ID (NCBI) 27033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2Y4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The zinc finger and BTB domain containing 32 (ZBTB32) protein, also known as testis zinc finger protein (TZFP) or repressor of GATA (ROG), is a transcription factor belonging to the BTB/POZ-ZF protein family. It is encoded by the Zbtb32 gene, located on proximal chromosome 7 in the mouse genome. ZBTB32 was shown to ensure proper regulation of meiosis during spermatogenesis by acting as a repressor of the androgen receptor (PMID: 22851713, 34337361).