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验证数据展示

  • WB analysis using 18700-1-AP

    WB analysis using 18700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18700-1-AP (ZBTB7A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 18700-1-AP

    IF Staining of MCF-7 using 18700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using ZBTB7A antibody (18700-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

ZBTB7A Polyclonal antibody

ZBTB7A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 18700-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, ELISA

别名

HIV-1 1st-binding protein 1, HIV 1 1st binding protein 1, FBI-1, FBI1, FBI 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293T cells, NCI-H1299 cells, U2OS cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18700-1-AP targets ZBTB7A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 7A
别名 HIV-1 1st-binding protein 1, HIV 1 1st binding protein 1, FBI-1, FBI1, FBI 1
计算分子量 61 kDa
观测分子量61-70 kDa
GenBank蛋白编号BC113511
基因名称 ZBTB7A
Gene ID (NCBI) 51341
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB7A, also named as LRF, FBI1, FBI-1, pokemon, ZBTB7, ZNF857A and DKFZp547O146, a member of the POK (POZ and Krüppel) family of transcription factors, plays a role in differentiation, oncogenesis, and adipogenesis (PMID: 35798238). ZBTB7A is involved in the instruction of early lymphoid progenitors to develop into B lineage by repressing T-cell instructive Notch signals. ZBTB7A is widely expressed in normal thymus and it is aberrantly overexpressed in human cancers (PMID: 28942243). ZBTB7A has 10 potential sumoylation sites located at lysine 61, 354, 371, 379, 383, 396, 486, 487, 536 and 539 (PMID: 20471975, 19471103). ZBTB7A has a predicted molecular weight of 61 kDa and the 75~80-kDa band could be the sumoylated form.

实验方案

Product Specific Protocols
WB protocol for ZBTB7A antibody 18700-1-APDownload protocol
IF protocol for ZBTB7A antibody 18700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Biomed Res Int

Obesity-Induced Upregulation of ZBTB7A Promotes Lipid Accumulation through SREBP1.

Authors - Jing-Ping Zhou
  • KD Validated
View Article