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ZBTB7B Polyclonal antibody

ZBTB7B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28127-1-AP

Print datasheet

Synonyms

c Krox, DKFZp686G01254, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B

验证数据展示

  • WB analysis using 28127-1-AP

    WB analysis using 28127-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28127-1-AP (ZBTB7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZBTB7B Ready-To-Use IHC Kit. ZBTB7B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28127-1-AP targets ZBTB7B in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen ZBTB7B fusion protein Ag27970 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 7B
Synonyms c Krox, DKFZp686G01254, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B
Calculated Molecular Weight 539 aa, 58 kDa
Observed Molecular Weight 70 kda
GenBank Accession NumberBC012070
Gene Symbol ZBTB7B
Gene ID (NCBI) 51043
RRIDAB_2881068
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15156
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB7B, also known as Zfp-67 or Zinc finger protein Th-POK, is a 539 amino acid protein, which localizes in nucleus. ZBTB7B as a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. ZBTB7B is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. The calcualted molecular weight of ZBTB7B is about 58 kDa, but modified protein is 70 kDa.

实验方案

Product Specific Protocols
WB protocol for ZBTB7B antibody 28127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols