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验证数据展示

  • WB analysis using 28127-1-AP

    WB analysis using 28127-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28127-1-AP (ZBTB7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ZBTB7B Polyclonal antibody

ZBTB7B Polyclonal Antibody for WB, ELISA
Cat No. 28127-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

c Krox, DKFZp686G01254, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ZBTB7B Ready-To-Use IHC Kit. ZBTB7B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28127-1-AP targets ZBTB7B in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原 ZBTB7B fusion protein Ag27970 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 7B
别名 c Krox, DKFZp686G01254, hcKrox, THPOK, ZBTB15, ZBTB7B, Zfp 67, ZFP67, Zinc finger protein 67 homolog, Zinc finger protein Th POK, ZNF857B
计算分子量 539 aa, 58 kDa
观测分子量 70 kda
GenBank蛋白编号BC012070
基因名称 ZBTB7B
Gene ID (NCBI) 51043
RRIDAB_2881068
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15156
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB7B, also known as Zfp-67 or Zinc finger protein Th-POK, is a 539 amino acid protein, which localizes in nucleus. ZBTB7B as a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. ZBTB7B is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. The calcualted molecular weight of ZBTB7B is about 58 kDa, but modified protein is 70 kDa.

实验方案

Product Specific Protocols
WB protocol for ZBTB7B antibody 28127-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols