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ZC3H12A Monoclonal antibody

ZC3H12A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F6B2

Cat no : 68616-1-Ig

Print datasheet

Synonyms

dJ423B22.1, FLJ23231, MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A

验证数据展示

  • WB analysis using 68616-1-Ig

    WB analysis using 68616-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68616-1-Ig (ZC3H12A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inHT-1376 cells, BxPC-3 cells, MDA-MB-468 cells, Raji cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H12A Ready-To-Use IHC Kit. ZC3H12A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68616-1-Ig targets ZC3H12A in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ZC3H12A fusion protein Ag13877 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type containing 12A
Synonyms dJ423B22.1, FLJ23231, MCP induced protein 1, MCPIP, MCPIP1, Ribonuclease ZC3H12A, RP3 423B22.1, ZC3H12A
Calculated Molecular Weight 599 aa, 66 kDa
Observed Molecular Weight66 kDa
GenBank Accession NumberBC005001
Gene Symbol ZC3H12A
Gene ID (NCBI) 80149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ5D1E8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H12A, also named as MCPIP or MCPIP1, is a 599 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12A localizes in the cytoplasm and nucleus. Increased expression of ZC3H12A is associated with ischemic heart disease. ZC3H12A has RNase activity and selectively degrades specific target mRNA species and modulates the immune response and inflammation by regulating the decay of specific mRNA molecules.

实验方案

Product Specific Protocols
WB protocol for ZC3H12A antibody 68616-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols