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ZC3H12D Monoclonal antibody

ZC3H12D Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1E2A2

Cat no : 68847-1-Ig

Print datasheet

Synonyms

MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95

验证数据展示

  • WB analysis using 68847-1-Ig

    WB analysis using 68847-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68847-1-Ig (ZC3H12D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inKarpas-422 cells, MJ cells, Raji cells, Ramos cells, Daudi cells, THP-1 cells
Planning an IHC experiment? We recommend our IHCeasy ZC3H12D Ready-To-Use IHC Kit. ZC3H12D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68847-1-Ig targets ZC3H12D in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ZC3H12D fusion protein Ag21770 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name zinc finger CCCH-type containing 12D
Synonyms MCP-induced protein 4, MCP induced protein 4, FLJ46041, EC:3.1.-.-, C6orf95
Calculated Molecular Weight 527 aa, 58 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC157832
Gene Symbol ZC3H12D
Gene ID (NCBI) 340152
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDA2A288
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H12D, also named as MCP induced protein 4, is a 527 amino acid protein, which contains one C3H1-type zinc finger and belongs to the ZC3H12 family. ZC3H12D exists as three isoforms and localizes in the cytoplasm. ZC3H12D may regulate cell growth likely by suppressing RB1 phosphorylation and serves as a tumor suppressor in certain leukemia cells. ZC3H12D is expressed in normal human lymphocytes but defective in some leukemia/lymphoma cell lines.

实验方案

Product Specific Protocols
WB protocol for ZC3H12D antibody 68847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols