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验证数据展示

  • WB analysis of HEK-293 using 68526-1-Ig

    WB analysis of HEK-293 using 68526-1-Ig

    WB result of ZC3H13 antibody (68526-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZC3H13 transfected HEK-293 cells.

  • WB analysis using 68526-1-Ig

    WB analysis using 68526-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68526-1-Ig (ZC3H13 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 68526-1-Ig

    IHC staining of human stomach cancer using 68526-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68526-1-Ig (ZC3H13 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ZC3H13 antibody included
  • Featured Product
  • KD/KO验证

ZC3H13 Monoclonal antibody

ZC3H13 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68526-1-Ig

产品说明书

CloneNo. 3E8C5

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0853, 3E8C5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HEK-293 cells, A549 cells, MCF-7 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZC3H13 Ready-To-Use IHC Kit. ZC3H13 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68526-1-Ig targets ZC3H13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ZC3H13 fusion protein Ag29132 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger CCCH-type containing 13
别名 KIAA0853, 3E8C5
计算分子量 197 kDa
观测分子量196-240 kDa
GenBank蛋白编号NM_001076788
基因名称 ZC3H13
Gene ID (NCBI) 23091
RRIDAB_3085234
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5T200
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZC3H13 antibody 68526-1-IgDownload protocol
IHC protocol for ZC3H13 antibody 68526-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols