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ZC3H18 Polyclonal antibody

ZC3H18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25807-1-AP

Print datasheet

Synonyms

NHN1, Nuclear protein NHN1, ZC3H18

验证数据展示

  • WB analysis of HepG2 using 25807-1-AP

    WB analysis of HepG2 using 25807-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25807-1-AP (ZC3H18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 25807-1-AP

    IP experiment of HeLa using 25807-1-AP

    IP result of anti-ZC3H18 (IP:25807-1-AP, 4ug; Detection:25807-1-AP 1:600) with HeLa cells lysate 3200 ug.

  • IHC staining of human tonsillitis using 25807-1-AP

    IHC staining of human tonsillitis using 25807-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25807-1-AP (ZC3H18 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman tonsillitis tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25807-1-AP is ZC3H18 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZC3H18 fusion protein Ag20481 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger CCCH-type containing 18
Synonyms NHN1, Nuclear protein NHN1, ZC3H18
Calculated Molecular Weight 953 aa, 106 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC050463
Gene Symbol ZC3H18
Gene ID (NCBI) 124245
RRIDAB_2721255
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86VM9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H18, also named as NHN1, has two CCCH zinc finger domains. It is not essential in bloodstream forms, but in an in vitro model of differentiation. ZC3H18 recruites the TREX protein for SEP1-mediated mRNA export. The gene ZC3H18 has some isoforms with calculated MW 160 kDa and 84 kDa. With phospho modification, the MW of ZC3H18 is about 150 kDa in WB detection.

实验方案

Product Specific Protocols
WB protocol for ZC3H18 antibody 25807-1-APDownload protocol
IHC protocol for ZC3H18 antibody 25807-1-APDownload protocol
IP protocol for ZC3H18 antibody 25807-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols