ZC3H4 Polyclonal antibody

ZC3H4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20041-1-AP

Print datasheet

Synonyms

C19orf7, KIAA1064, ZC3H4



经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20041-1-AP targets ZC3H4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger CCCH-type containing 4
Synonyms C19orf7, KIAA1064, ZC3H4
Calculated Molecular Weight 140 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberNM_015168
Gene Symbol ZC3H4
Gene ID (NCBI) 23211
RRIDAB_10667456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.

实验方案

Product Specific Protocols
WB protocol for ZC3H4 antibody 20041-1-APDownload protocol
IHC protocol for ZC3H4 antibody 20041-1-APDownload protocol
IF protocol for ZC3H4 antibody 20041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Biol Macromol

CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression

Authors - Zhongqiu Zhou
mouseWB,IF

FASEB J

Silica-induced initiation of circular ZC3H4 RNA/ZC3H4 pathway promotes the pulmonary macrophage activation.

Authors - Xiyue Yang
humanWB

Cell Biosci

A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation.

Authors - Juan Yin
mouseWB

Lab Invest

Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition.

Authors - Rong Jiang
mouseWB,IF

Toxicol Appl Pharmacol

ZC3H4 promotes pulmonary fibrosis via an ER stress-related positive feedback loop.

Authors - Hammad Ghafoor
humanWB,IF

Environ Toxicol Pharmacol

ZC3H4 mediates silica-induced EndoMT via ER stress and autophagy.

Authors - Rong Jiang