验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IF | See 3 publications below |
产品信息
20041-1-AP targets ZC3H4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger CCCH-type containing 4 |
| 别名 | C19orf7, KIAA1064, ZC3H4 |
| 计算分子量 | 140 kDa |
| 观测分子量 | 130-140 kDa |
| GenBank蛋白编号 | NM_015168 |
| 基因名称 | ZC3H4 |
| Gene ID (NCBI) | 23211 |
| RRID | AB_10667456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UPT8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IHC protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IF protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression | ||
FASEB J Silica-induced initiation of circular ZC3H4 RNA/ZC3H4 pathway promotes the pulmonary macrophage activation. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. | ||
Lab Invest Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition. | ||
Toxicol Appl Pharmacol ZC3H4 promotes pulmonary fibrosis via an ER stress-related positive feedback loop. | ||
Environ Toxicol Pharmacol ZC3H4 mediates silica-induced EndoMT via ER stress and autophagy. |
