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ZC3HC1 Polyclonal antibody

ZC3HC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13148-1-AP

Print datasheet

Synonyms

Zinc finger C3HC-type protein 1, Nuclear-interacting partner of anaplastic lymphoma kinase, Nuclear-interacting partner of ALK, NIPA, HSPC216

验证数据展示

  • WB analysis using 13148-1-AP

    WB analysis using 13148-1-AP

    A431 cell lysates were subjected to SDS PAGE followed by western blot with 13148-1-AP (ZC3HC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13148-1-AP

    IHC staining of human stomach using 13148-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13148-1-AP (ZC3HC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, mouse skeletal muscle tissue, mouse heart tissue, mouse lung tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13148-1-AP targets ZC3HC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ZC3HC1 fusion protein Ag3744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger, C3HC-type containing 1
Synonyms Zinc finger C3HC-type protein 1, Nuclear-interacting partner of anaplastic lymphoma kinase, Nuclear-interacting partner of ALK, NIPA, HSPC216
Calculated Molecular Weight 502 aa, 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC028917
Gene Symbol ZC3HC1
Gene ID (NCBI) 51530
RRIDAB_2217514
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86WB0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZC3HC1 antibody 13148-1-APDownload protocol
IHC protocol for ZC3HC1 antibody 13148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Proteomics

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Authors - Hao-Yu Li
View Article