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ZCCHC2 Polyclonal antibody

ZCCHC2 Polyclonal Antibody for IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Conjugate

Unconjugated

Cat no : 25741-1-AP

Print datasheet

Synonyms

KIAA1744, C18orf49

验证数据展示

  • IP experiment of HeLa using 25741-1-AP

    IP experiment of HeLa using 25741-1-AP

    IP result of anti-ZCCHC2 (IP:25741-1-AP, 4ug; Detection:25741-1-AP 1:500) with HeLa cells lysate 1800 ug.

经过测试的应用

Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25741-1-AP targets ZCCHC2 in IP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, ELISA Application Description
Tested Reactivity human
Immunogen ZCCHC2 fusion protein Ag22744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger, CCHC domain containing 2
Synonyms KIAA1744, C18orf49
Calculated Molecular Weight 1178 aa, 126 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC006340
Gene Symbol ZCCHC2
Gene ID (NCBI) 54877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0B9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZCCHC2 is a poorly characterized protein of 126 kDa with long intrinsically disordered regions, a PX domain, and a CCHC-type zinc-finger (ZnF) domain. ZCCHC2 is distantly related to ZCCHC14 but lacks the SAM domain that is known to interact with the CNGGN pentaloop in 1E and PRE. ZCCHC2 is a critical host factor for K5. ZCCHC2 recruits the terminal nucleotidyl transferase TENT4 to elongate poly(A) tails with mixed sequences, delaying deadenylation. The observed molecular weight is consistent with what has been described in the literature (PMID: 37413987).

实验方案

Product Specific Protocols
IP protocol for ZCCHC2 antibody 25741-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols