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- KD/KO Validated
HIP14; ZDHHC17 Polyclonal antibody
HIP14; ZDHHC17 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human kidney tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15465-1-AP targets HIP14; ZDHHC17 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HIP14; ZDHHC17 fusion protein Ag7763 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | zinc finger, DHHC-type containing 17 |
| Synonyms | DHHC 17, HIP 14, HIP 3, HIP14, HIP14; ZDHHC17, HIP3, HSPC294, Huntingtin yeast partner H, HYPH, KIAA0946, Palmitoyltransferase ZDHHC17, ZDHHC17 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC050324 |
| Gene Symbol | ZDHHC17 |
| Gene ID (NCBI) | 23390 |
| RRID | AB_2217690 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IUH5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIP14; ZDHHC17 antibody 15465-1-AP | Download protocol |
| IHC protocol for HIP14; ZDHHC17 antibody 15465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
mBio Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection
| ||
J Cell Sci Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus | ||
Mol Cell Endocrinol ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells |
