验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human kidney tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15465-1-AP targets HIP14; ZDHHC17 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | HIP14; ZDHHC17 fusion protein Ag7763 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, DHHC-type containing 17 |
| 别名 | ZDHHC17, HIP 14, EC:2.3.1.225, EC:2.3.1.-, DHHC domain-containing cysteine-rich protein 17 |
| 计算分子量 | 73 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC050324 |
| 基因名称 | ZDHHC17 |
| Gene ID (NCBI) | 23390 |
| RRID | AB_2217690 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IUH5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Post-translational modification by the lipid palmitate is crucial for the correct targeting and function of many proteins. Polyglutamine expansions of huntingtin protein are responsible for the Huntington neurological disorder. ZDHHC17 gene encodes palmitoyl transferase huntingtin interacting protein (HIP14) which regulates palmitoylation and distribution of hungtingtin. The latter is implicated in the formation of inclusion bodies and enhanced neuronal toxicity. HIP14 was also shown to control neurotransmitter release.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIP14; ZDHHC17 antibody 15465-1-AP | Download protocol |
| IHC protocol for HIP14; ZDHHC17 antibody 15465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
mBio Identification of ZDHHC17 as a Potential Drug Target for Swine Acute Diarrhea Syndrome Coronavirus Infection
| ||
J Cell Sci Activity-dependent post-translational regulation of palmitoylating and depalmitoylating enzymes in the hippocampus | ||
Mol Cell Endocrinol ZDHHC17 participates in the pathogenesis of polycystic ovary syndrome by affecting androgen conversion to estrogen in granulosa cells |
