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ZEB1 Monoclonal antibody, PBS Only

ZEB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H1F1

Cat no : 66279-1-PBS

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Synonyms

AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A

验证数据展示

  • WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

产品信息

66279-1-PBS targets ZEB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ZEB1 fusion protein Ag17118 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name zinc finger E-box binding homeobox 1
Synonyms AREB6, BZP, DELTA EF1, delta EF1,, Negative regulator of IL2, NIL 2 A, NIL 2 A zinc finger protein, NIL 2A, NIL2A, TCF 8, TCF8, Transcription factor 8, ZEB, ZEB1, ZFHEP, ZFHX1A
Calculated Molecular Weight 1125 aa, 124 kDa
Observed Molecular Weight 190-200 kDa
GenBank Accession NumberBC112392
Gene Symbol ZEB1
Gene ID (NCBI) 6935
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37275
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZEB1(zinc-finger-enhancer binding protein 1), which is a members of the Zincfinger-Homeobox (ZFH)-family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene . Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post phosphorylation of protein is about 190-210 kDa.