验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human gliomas tissue, human lung cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14026-1-AP targets ZEB2 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP, RIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish |
| 免疫原 | ZEB2 fusion protein Ag5171 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger E-box binding homeobox 2 |
| 别名 | KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B |
| 计算分子量 | 136 kDa |
| 观测分子量 | 150-170 kDa |
| GenBank蛋白编号 | BC060819 |
| 基因名称 | ZEB2 |
| Gene ID (NCBI) | 9839 |
| RRID | AB_2878001 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O60315 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZEB2 antibody 14026-1-AP | Download protocol |
| IHC protocol for ZEB2 antibody 14026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation | ||
Nat Commun Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. | ||
Cancer Lett A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation | ||
Oncotarget Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression. | ||
Cell Death Dis PIM1 mediates epithelial-mesenchymal transition by targeting Smads and c-Myc in the nucleus and potentiates clear-cell renal-cell carcinoma oncogenesis. |