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验证数据展示

  • IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

    IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

  • IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

    IHC staining of human renal cell carcinoma using 82020-1-RR (same clone as 82020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 82020-1-RR (ZEB2 antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

    IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

    IF Staining of rat brain using 82020-1-RR (same clone as 82020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ZEB2 antibody (82020-1-RR, Clone: 1K22 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82020-1-RR

    Affinity and Kinetic Characterization of 82020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82020-1-RR against Human ZEB2 were performed. The affinity constant is 0.682 nM.

  • WB analysis using 82020-1-RR (same clone as 82020-1-PBS)

    WB analysis using 82020-1-RR (same clone as 82020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82020-1-RR (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82020-1-PBS in a different storage buffer formulation.

ZEB2 Recombinant antibody, PBS Only

ZEB2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 82020-1-PBS

产品说明书

CloneNo. 1K22

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

SIP1, 1K22, HRIHFB2411, KIAA0569, SIP 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82020-1-PBS targets ZEB2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 ZEB2 fusion protein Ag5171 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger E-box binding homeobox 2
别名 SIP1, 1K22, HRIHFB2411, KIAA0569, SIP 1
计算分子量 136 kDa
观测分子量 140-150 kD
GenBank蛋白编号BC060819
基因名称 ZEB2
Gene ID (NCBI) 9839
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO60315
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. PMID: 27659015 )