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验证数据展示

  • WB analysis of Jurkat using 68258-1-Ig

    WB analysis of Jurkat using 68258-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of LNCaP using 68258-1-Ig

    WB analysis of LNCaP using 68258-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68258-1-Ig (ZER1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68258-1-Ig

    IF Staining of HepG2 using 68258-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZER1 antibody (68258-1-Ig, Clone: 1H9G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

ZER1 Monoclonal antibody

ZER1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68258-1-Ig

产品说明书

CloneNo. 1H9G7

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68258-1-Ig targets ZER1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 ZER1 fusion protein Ag10040 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 zer-1 homolog (C. elegans)
别名 C9orf60, Hzyg, Protein zer 1 homolog, RP11 545E17.4, zer 1 homolog (C. elegans), ZER1, ZYG, Zyg 11 homolog B like protein, ZYG11BL
计算分子量 766 aa, 88 kDa
观测分子量 80-88 kDa
GenBank蛋白编号BC052563
基因名称 ZER1
Gene ID (NCBI) 10444
RRIDAB_2935343
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z7L7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ZER1 antibody 68258-1-IgDownload protocol
IF protocol for ZER1 antibody 68258-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols