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验证数据展示

  • WB analysis using 22056-1-AP

    WB analysis using 22056-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22056-1-AP (ZFP161 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ZFP161 / ZBTB14 Polyclonal antibody

ZFP161 / ZBTB14 Polyclonal Antibody for WB, ELISA
Cat No. 22056-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hZF5, ZBTB14, ZF5, Zfp 161, Zfp 5, ZFP161, Zinc finger protein 5 homolog, ZNF478

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, L02 cells
Planning an IHC experiment? We recommend our IHCeasy ZFP161 Ready-To-Use IHC Kit. ZFP161 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22056-1-AP targets ZFP161 / ZBTB14 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ZFP161 / ZBTB14 fusion protein Ag17082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger protein 161 homolog (mouse)
Synonyms hZF5, ZBTB14, ZF5, Zfp 161, Zfp 5, ZFP161, Zinc finger protein 5 homolog, ZNF478
Calculated Molecular Weight 449 aa, 51 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC110519
Gene Symbol ZFP161
Gene ID (NCBI) 7541
RRIDAB_3085683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43829
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene. The calculated MW of ZFP161 is 52 kDa, 22056-1-AP can detect a band around 55 kDa.

实验方案

Product Specific Protocols
WB protocol for ZFP161 / ZBTB14 antibody 22056-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols