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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83909-4-RR (same clone as 83909-4-PBS)

    WB analysis using 83909-4-RR (same clone as 83909-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83909-4-RR (ZFP161 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83909-4-RR (same clone as 83909-4-PBS)

    FC experiment of MCF-7 using 83909-4-RR (same clone as 83909-4-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ZFP161 / ZBTB14 Recombinant antibody (83909-4-RR, Clone:240853B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83909-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83909-4-RR

    Affinity and Kinetic Characterization of 83909-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83909-4-RR against Human ZFP161 / ZBTB14 were performed. The affinity constant is below 1 pM.

ZFP161 / ZBTB14 Recombinant antibody, PBS Only

ZFP161 / ZBTB14 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83909-4-PBS

产品说明书

CloneNo. 240853B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ZFP161 Ready-To-Use IHC Kit. ZFP161 primary antibody included.

产品信息

83909-4-PBS targets ZFP161 / ZBTB14 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 ZFP161 / ZBTB14 fusion protein Ag16125 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 zinc finger protein 161 homolog (mouse)
别名 ZFP161, Zfp 5, Zfp 161, ZF5, ZBTB14
计算分子量 449 aa, 51 kDa
观测分子量 52 kDa
GenBank蛋白编号BC110519
基因名称 ZFP161
Gene ID (NCBI) 7541
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO43829
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZBTB14, also named as :ZFP161, ZNF478, is a 449 amino acid protein, which interacts with ZBTB21. ZBTB14, is a transcriptional activator of the dopamine transporter (DAT), binding it's promoter at the consensus sequence 5'-CCTGCACAGTTCACGGA-3'. ZBTB14 Binds to 5'-d(GCC)(n)-3' trinucleotide repeats in promoter regions and acts as a repressor of the FMR1 gene.