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Rabenosyn 5 Polyclonal antibody

Rabenosyn 5 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (2)

Conjugate

Unconjugated

Cat No : 22218-1-AP

Print datasheet

Synonyms

110 kDa protein, Rabenosyn 5, ZFYVE20

验证数据展示

  • WB analysis of K-562 using 22218-1-AP

    WB analysis of K-562 using 22218-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22218-1-AP

    IHC staining of human testis using 22218-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 22218-1-AP

    IHC staining of human testis using 22218-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inK-562 cells, A549 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22218-1-AP is Rabenosyn 5 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA Application Description
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Rabenosyn 5 fusion protein Ag17554 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger, FYVE domain containing 20
Synonyms 110 kDa protein, Rabenosyn 5, ZFYVE20
Calculated Molecular Weight 784 aa, 89 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC106940
Gene Symbol ZFYVE20
Gene ID (NCBI) 64145
RRIDAB_11182179
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1K0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Rabenosyn 5 antibody 22218-1-APDownload protocol
IHC protocol for Rabenosyn 5 antibody 22218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

EMBO Rep

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

Authors - Ye Chen
View Article
humanWB, IHC

J Mol Biol

Identification of New Interactions between Endolysosomal Tethering Factors.

Authors - Zsófia Simon-Vecsei
View Article
mouseWB,IF,IP

Int J Mol Sci

Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage

Authors - Masato Mashimo
View Article
humanWB,IF

Nat Commun

Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic

Authors - Seiichi Koike
View Article