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验证数据展示

  • WB analysis of K-562 using 22218-1-AP

    WB analysis of K-562 using 22218-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22218-1-AP

    IHC staining of human testis using 22218-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 22218-1-AP

    IHC staining of human testis using 22218-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

Rabenosyn 5 Polyclonal antibody

Rabenosyn 5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22218-1-AP

产品说明书

引用文献(4)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (2)

别名

110 kDa protein, Rabenosyn 5, ZFYVE20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, mouse brain tissue, mouse heart tissue, mouse kidney tissue, mouse skeletal muscle tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22218-1-AP targets Rabenosyn 5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IP, IF, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Rabenosyn 5 fusion protein Ag17554 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 20
别名 110 kDa protein, Rabenosyn 5, ZFYVE20
计算分子量 784 aa, 89 kDa
观测分子量 110 kDa
GenBank蛋白编号BC106940
基因名称 Rabenosyn 5
Gene ID (NCBI) 64145
RRIDAB_11182179
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1K0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Rabenosyn 5 antibody 22218-1-APDownload protocol
IHC protocol for Rabenosyn 5 antibody 22218-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

EMBO Rep

Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.

Authors - Ye Chen
View Article
humanWB, IHC

J Mol Biol

Identification of New Interactions between Endolysosomal Tethering Factors.

Authors - Zsófia Simon-Vecsei
View Article
mouseWB,IF,IP

Int J Mol Sci

Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage

Authors - Masato Mashimo
View Article
humanWB,IF

Nat Commun

Rab GTPases and phosphoinositides fine-tune SNAREs dependent targeting specificity of intracellular vesicle traffic

Authors - Seiichi Koike
View Article