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Protrudin Polyclonal antibody

Protrudin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12680-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, ELISA and More (3)

ZFYVE27, Zinc finger FYVE domain-containing protein 27, SPG33, Spastic paraplegia 33 protein, RP11 459F3.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, monkey
免疫原 Protrudin fusion protein Ag3369 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 27
别名 ZFYVE27, Zinc finger FYVE domain-containing protein 27, SPG33, Spastic paraplegia 33 protein, RP11 459F3.2
计算分子量 411 aa, 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC030621
基因名称 ZFYVE27
Gene ID (NCBI) 118813
RRIDAB_10640298
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T4F4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

实验方案

Product Specific Protocols
WB protocol for Protrudin antibody 12680-1-APDownload protocol
IHC protocol for Protrudin antibody 12680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.

Authors - Yael Elbaz-Alon
ratWB

Nat Commun

Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.

Authors - Veselina Petrova
humanWB

Nat Commun

Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.

Authors - Parikshit Bagchi
humanWB,IF,IP

Proc Natl Acad Sci U S A

Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.

Authors - Chang Jaerak J
  • KD Validated
human

Cell Mol Life Sci

ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.

Authors - James W Connell
humanWB

Cell Mol Life Sci

Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.

Authors - Amita Arora
  • KD Validated
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