• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ZFYVE28 Polyclonal antibody

ZFYVE28 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25360-1-AP

Print datasheet

Synonyms

Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2

验证数据展示

  • WB analysis using 25360-1-AP

    WB analysis using 25360-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25360-1-AP (ZFYVE28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25360-1-AP

    IF Staining of HepG2 using 25360-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 25360-1-AP

    IF Staining of HeLa using 25360-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ZFYVE28 fusion protein Ag19796 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger, FYVE domain containing 28
Synonyms Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2
Calculated Molecular Weight 887 aa, 96 kDa
Observed Molecular Weight 96 kDa
GenBank Accession NumberBC137309
Gene Symbol ZFYVE28
Gene ID (NCBI) 57732
RRIDAB_3085790
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCC9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.

实验方案

Product Specific Protocols
WB protocol for ZFYVE28 antibody 25360-1-APDownload protocol
IF protocol for ZFYVE28 antibody 25360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols